บทคัดย่องานวิจัย

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Ascorbate metabolism in two cultivars of postharvest spinach differing in their senescence rates

D.M. Hokges and C.F. Forney

Proceedings of 26th International Horticultural Congress. Volume of Abstract . Toronto, Canada, 11-17 August, 2002. Abstract S09-P-174. pp. 264.

2002

บทคัดย่อ

Ascorbate Metabolism in Two Cultivars of Postharvest Spinach Differing in Their Senescence Rates

We have shown that rapidly declining levels of ascorbate (vitamin C) are as sociated with advancing senescence and postharvest quality loss. To fruther explore the association between ascorbate metabolism and senescence, two cultivars of spinach (Spinacia oleracea L.) previously shown to differ in their postharvest senescence ratres were grown under controlled conditions (18 oC, 14:10 L:D) and leaves harvested 6 weeks after planting. Detached leaves of Spokane F1 (relatively fast senescence rate) and BJ412 Sponsor (relatively slow senescence rate) were bagged and placed in the dark at 10 oC. Samples were removed on days 0, 7, 14, 21, 28, and 35 and analyzed for activities of galactono-gamma-lactone dehydrogenase, ascorbate reductase, ascorbate peroxidase and levels of ascorbate (reduced and oxidized) and malondialdehke (estimator of lipid peroxidation). Differences in ascorbate metabolic properties between these two cultivars will be discussed.