X-ray microanalysis of apples treated with kaolin indicates wax-embedded particulates in the cuticle
Eric Curry , Donald Baer and James Young
ISHS Acta Horticulturae 636: 497-503.
2004
บทคัดย่อ
‘Gala’ apples trees were treated with kaolin (Surround) in mid-July, and fruit were sample 1 and 7 days after treatment. Apples were gently rinsed with cold deionized water to remove looose surface material. Peel tissue about 0.3 mm thick was excised wirh a razor and flash frozen on an aluminum block held at –190 oC, freeze-dried, and coated with a thin film of carbon. Samples were examined using a LEO 982 Field Emission Scanning Electron Microscope (FESEM) equipped with an Oxford ISIS energy dispersive x-ray microanalysis system capable of light element detection. Qualitative secondary electron imaging combined with quantitative elemental compositional analyses indicated kaolin particles may become embedded in the cuticle between individual was platelets. Effects on cuticle integrity will be discussed.