บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Senescence of cut chrysanthemum regulated by 6-BA, ABA pretreatment

Sumei Chen and Weiming Guo

Ornamental Dept. of Horticultural College of Nanjing Agricultural Univ., Jiangsu, P.R. China 210095

2002

บทคัดย่อ

Senescence of Cut Chrysanthemum Regulated by 6-BA, ABA Pretreatment

Soluble protein content of leaves in cut chrysanthemum ‘Shi Yuehuang’, SOD activity, POD activity of leaves and florets were determined in cut chrysanthemum ‘Si Jihuang’. The results showed that 6-BA, ABA pretreatment delayed or enhanced the senescence of cut chrysanthemum by inhibiting or stimulating the degradation of soluble proteins, respectively. 6-BA pretreatment increased and maintained the higher SOD activity in leaves at stage 4 and stage 5, but ABA pretreatment depressed SOD activity. The POD activity increased during whole vase duration, and ABA pretreatment stimulated it, POD activity under 6-BA pretreatment remained lower level than that of ABA pretreatment stimulated it, POD activity under 6-BA pretreatment remained lower level than that of ABA pretreatment. SDS-PAGE (12.5% gel) profiles of proteins of leaves in cut chrysanthemum ‘Shi Yuehuang’ showed that ABA pretreatment could induced two new peptides (Rf 0.486, 0.638) relating to senescence at stage 4. SDS-PAGE (12.5%gel) profiles of proteins of leaves in cut chrysanthemum ‘Si Jihuang’ at wilting stage (stage 5) indicated contrary regulations by 6-BA, ABA pretreatment, ABA pretreatmentgreatly enhanced the expression of peptide at Rf 0.309 which was inhibited by 6-BA, other three new peptides (Rf 0.382, 0.418, 0.455) induced by ABA pretreatment were also inhibited by 6-BA pretreatment, ABA pretreatment caused the loss of three peptieds (Rf 0.291, 0.327, 0.345) , but 6-BA pretreatment retarded the degradation of those three peptides. It was concluded that these proteins might be responsible for the early senescence of leaves. Different regulation of expressions of SOD, POD isozyme by 6-BA, ABA was also observed, PAGE (7.5% gel) profiles showed that SOD isozyme bands in leaves and florets pretreated by 6-BA were deeply stained than those of ABA pretreatment, but PAGE profiles of POD isozyme in florets showed opposite characteristic of SOD stain.