บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Use of molecular markers to improve cut flower longevity in carnation

Laura De Benedetti, De Benedetti, L., Burchi, G., Bruna, S., Mercuri, A. and Schiva, T.

Acta Hort. (ISHS) 624:343-348.

2002

บทคัดย่อ

Use of Molecular Markers to Improve Cut Flower Longevity in Carnation

Postharvest lognevity is one of the most important traits considered by the breeders of carnation. The availabillity of fast and simple methods for the early screening of F1 progenies could be very important to improve the efficiency of the breeding programs. In fact, we have to consider that it takes almost two years to evaluate flower longevity in the progenies, starting from the F1 seeds. In this work, RAPD analysis was used for the indentification of molecular markers associated with flower vase life. These markers could then be used for the early screening of a population with a long vase life. Two cultivars (Roland and Milady) and their F1 and Backcross offspring were analyzed. The flowers of Roland showed a very long vase life with a very low ethylene production, whereas in Mildy the early release of ethylene by the flowers promoted and accelerated flower senescence. Our previous studies on the segregation of the character in these progenies indicated that flower vase life is probably a complex quantitative trait in carnation, involving more than a single gene or mechanism, and that this character is controlled by genes showing predominantly additive effects. Sixty random primers were initially tested on a few genotypes. DNAs from 73 F1 and 36 Backcross offspring were then analysed with 10 selected primers. For each of RAPD band tested, the progenies were divided into two groups according to the similarity of the band pattern with Roland or Milady. The statistical significance of the differences in vase life between the two groups was evaluated. As a result, several bands resulted to discriminate significatively a more longeve population. Statistical analysis showed a positive correlation between the score of each progeny (number of RAPD bands similar to Roland) and flower longevity. These markers will be tested in other crosses in order to verify their general use for the assisted selection of flower vase life.