Analysis of aflatoxin B1 in corn using capillary electrophoresis with laser-induced fluorescence detection.
MARAGOS, C.M. and GREER, J.I.
Journal of Agricultural and Food Chemistry 45 : 4337-4341.
1997
บทคัดย่อ
A capillary electrophoresis (CE) method, developed for the quantitation of AFB1 in corn, is described. The instrumentation can be assembled easily from readily available components and takes advantage of the native fluorescence of AFB1. Corn was extracted and aflatoxins were isolated using either silica column of affinity column cleanup procedures. Following cleanup, the analysis of each sample required 15 min. The CE method was compared to an established HPLC method for the determination of AFB1 in corn. The limit of detection by CE was 0.5 mg/kg, with a useful range of 1-100 mg/kg in spiked corn. Recovery of AFB1 averaged 85% over the range of 1-50 mg/kg (89% by HPLC). Forty naturally contaminated corn samples examined by using both methods showed good agreement.