Trichodiene as a volatile marker for trichothecenes biosynthesis.
JELEN, H., LATUSZIETKIEWICZ, D., WASOWICZ, E. and KAMINSKI, E.
Journal of Microbiological Methods 31 : 45-49.
1997
บทคัดย่อ
The ability of Fusarium strains, belonging to five different species, to produce volatile compounds and trichothecenes was monitored by GC/MS. Isolates were grown for 21 days on autoclaved wheat kernels. The volatiles were collected after 3 and 21 days on Tenax GR traps using a dynamic headspace method, and immediately after volatiles collection the cultures were checked for trichothecenes. Of nine analysed strains, seven were toxigenic and produced both types A and B trichothecenes. Among volatile metabolites, sesquiterpene hydrocarbon trichodiene was produced exclusively by toxigenic strains representing four species and was detected in all toxigenic strains after 3 days incubation. Trichodiene presence can serve as an indicator for undergoing trichothecene biosynthesis.