Studies on somaclonal variants for resistance to scab in bread wheat (Triticum aestivum L.) through in vitro selection for tolerance to deoxynivalenol.
YANG, A.P., YANG, X.Y. and HUANG, D.C.
Euphytica 101: 213 – 219.
1998
บทคัดย่อ
Immature embryos of two wheat varieties, a scab resistant variety Sumai 3 and a susceptible variety Mianyang 11, and their reciprocal F – 1 hybrids were cultured on MS medium supplemented with DON for callus induction. The percentage of callus fromation of reistant variety Sumai 3 on induction medium containing DON was higher than that of susceptible variety Mianyang 11. Regeneration of DON tolerant calli on DON containing differentiation medium differed signigicantly between Sumai 3 and Mianyang 11. Averaged across the DON tolerant calli of two varieties and their reciprocals, regeneration of DON tolernat calli was decreased 3 fold on DON containing medium. Several resistant lines from progenies of regenerated plants from DON tolerant celli were obtained and these somaclonal lines had lower disease scoring, shorter plans and better yield components than Sumai 3.