บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

One – step solid – phase extraction cleanup and fluorometric analysis of deoxynivalenol in grains.

MALONE, B.R., HUMPHREY, C.W., ROMER, T.R. and RICHARD, J.L.

Journal of AOAD International 81: 448 – 452.

1998

บทคัดย่อ

One – step solid – phase extraction cleanup and fluorometric analysis of deoxynivalenol in grains.

A rapid, quantitative method was developed for the determination of DON in wheat, barley, corn, wheat middlings, wheat flour, bran, malted barley and oats. Samples are ground and extracted with acetonitrile – water (86+14). A portion of the wxtract is cleaned up by passage through a MycoSep No. 225 column, evaporated to dryness, and derivatised with zirconyl nitrate and ethylenediamine in methanol. The resulting fluorescent derivative of DON is identified quantified with a calibrated fluorometer containing a broad wavelength pulsed xenon light source. This method quantified DON concentrations from 0.5 to 50 mg/kg without dilution and was linear when applied to samples of wheat spiked with DON at 0.5 – 50 mg/kg.