Analysis of Fusarium toxins in maize and wheat using thin layer chromatography.
SCHAAFSMA, A.W., NICOL, R.W., SAVARD, M.E., SINHA, R.C., REID, L.M. and ROTTINGHAUS, G.
Mycopathologia 142: 107-113.
1998
บทคัดย่อ
TLC methods for identifying and quantifying DON, FB1 and ZEA in grain samples were compared to ELISA and HPLC methods to determine the reliability of the less expensive TLC. There was very good agreement between levels of DON measured by TLC and competitive-direct ELISA, and between levels of FB1 measured by TLC and HPLC, over a wide range of concentrations. Correlation coefficients (Pearson’s) were 0.978, 0.914 and 0.953 for DON in maize, DON in wheat and FB1 in maize, respectively. A lower correlation coefficient was obtained when ZEA was quantified by TLC and HPLC. Possible reasons for this are discussed.