ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

A reliable field-inoculation method for identifying aflatoxin-resistant mize.

ZHANG, Y.D., SIMONSON, J.G., WANG, G.L., KANG, M.S. and MORRIS, H.F.

Cereal Research Communications 26: 245-251.

1998

บทคัดย่อ

The lack of a reliable field inoculation method is a significant impediment to maize breeders’ success in developing aflatoxin resistant hybrids. Previously available inoculation methods resulted in extremely high coefficients of variation for aflatoxin. This study evaluated four non-injury inoculation methods: needle-in-silk channel method involving injection of Aspergillus flavus conidial suspension into an ear through silk channel with a syringe; toothpick-in-silk channel (TSC) involving insertion of a Czapek medium-covered, conidia-laden toothpick in silk channel of an ear; toothpick-under-husk is similar to TSC, expect that Czapek-covered, conidia-laden toothpick is places on the surface of kernels by making an incision in the husk in the middle of an ear; and bag inoculation involving placement on an ear of an inoculation bag (a shoot bag) at the bottom of which was placed 5 mL Czapek medium that is covered with conidia. The toothpick-under-husk inoculation method had a substantially reduces coefficient of variation and was judged most reliable for evaluating aflatoxin concentration differences.