Stability and determination of aflatoxins by high-performance liquid chromatography with amperometric detection.
EKIZALDE-GONZALEZ,M.P., MATTUSCH, J. and WENNRICH, R.
Journal of Chromatographic A 828: 439-444.
1998
บทคัดย่อ
A method is described for the separation and identification of AFB1, B2, G1 ad G2 based on reversed phase HPLC with amperometric detection with a glassy carbon electrode at a constant potential of 1.4 V. The chromatography was performed on a PAH-Baker column with a ternary mobile phase containing methanol, acetonitrile and aqueous LiCIO4 electrolyte. AFG1 showed the highest electroactivity. Calibration curves of AFG1 and B1 were linear up to 0.2 and 0.3 mmol/L, respectively. Sensitivity varied between 7 and 10 ng for the different aflatoxins. The combination of different HPLC detectors in the analysis of these compounds was applied to investigate the stability of AFG1 and B2.