ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

A rapid TLC-scanning method for the determination of patulin in apple products.

VERO, S., VAZQUEZ, A., CERDEIRAS, M.P. and SOUBES, M.

JPP-Journal of Planar Chromatography-Modern TLC 12: 172-174.

1999

บทคัดย่อ

A method is described for the quantification of patulin in apples, apple concentrate and apple juice.  Patulin was extracted with ethyl acetate and the extracted solution was further cleaned with 1.5% sodium carbonate solution before separation on silica gel layers with toluene-ethyl acetate-formic acid (6+3+1) as mobile phase.  The chromatogram was scanned in reflectance mode at lambda = 275 nm, or at lambda = 412 nm if patulin was revealed by use of NBTH as detection reagent. The detection limit was 30 ng per spot when MBTH was used for detection and 50 ng per spot for direct UV quantification.  The average recovery of the method was 78% for quantities between 50 and 150 ug/kg.