บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Analysis of patulin in apple juice by diphasic dialysis extraction with in situ acylation and mass spectrometric determination.

SHEU, F. and SHYU, Y.T.

Jounal of Agricultural and Food Chemistry 47: 2711-2714.

1999

บทคัดย่อ

Analysis of patulin in apple juice by diphasic dialysis extraction with in situ acylation and mass spectrometric determination

A procedure combining diphasic dialysis extraction with in situ acylation and GC/MS determination was developed for detection and quantification of  patulin in apple juice.  Apple juice samples spiked with 4-N<N-dimethyl aminopyridine were dialysed using methane chloride and  acetic anhydride inside dialysis tubing.  Patulin was dervatised into its acetate and collected in the tubing after diphasic dialysis.  Patulin was directly determined using GC/MS with the selective ion monitoring mode without further concentration and clkeanup steps.  The calibration curve was linear in the range 10-250ug/L and the limit of quantification was 10/ug/L. Levels of patulin ranging from 0 to 107.2 ug/L with 77-109% recovery were found in 10 samples.