บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Identification of mimotope peptides which bind to the mycotoxin deoxynivalenolspceific monoclonal antibody.

YUAN, Q.P., PESTKA, J.J., HESPENHEIDE, G.M., KUHN, L.A., LINZ, J.E. and HART, L.P.

Applied and Environmental Microbiology 65: 3279-3286.

1999

บทคัดย่อ

Identification of mimotope peptides which bind to the mycotoxin deoxynivalenolspceific monoclonal antibody.

Monoclonal antibody 6F5 (mAb 6F5) which recognises DON was used to select for peptides that mimic the mycotoxin by employing a library of filamentous phages that have random 7-mer peptides on their surfaces.  Two phage clones were selected from the random  peptide phage-displayed library and subjected to a competitive ELISA.  Results suggested that the two phage displayed peptides bound to mAb 6F5 specifically at the DON binding site.  The potential of using a phage clone selected from the as an immunochemical reagent in a DON immunoassay was evaluted with a DON-spiked wheat extract.