บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Determination of nivalenol and deoxynivalenol in wheat using liquid chromatography-mass spectrometry with negative ion atmospheric pressure chemical ionisation.

RAZZAZI-FAZELI, E., BOHM, J. and LUF, W.

Journal of Chromatography A 854: 45-55.

1999

บทคัดย่อ

Determination of nivalenol and deoxynivalenol in wheat using liquid chromatography-mass spectrometry with negative ion atmospheric pressure chemical ionisation.

A new, rapid and sensitive method has been developed for the determination of nivalenol (NIV) and DON by using HPLC in combination with an atmospheric pressure chemical ionisation (APCI) – interface and a single quadrupole mass spectrometer.  The extraction was carred out using acetonitrile-water.  A  two step purification was then applied on two different Mycosep cleanup columns.  A modified, raped and isocratic HPLC method was used combined with a negative ion APCI-MS for the separation and quantitative determination of NIV and DON in wheat extract.  A reversed phase C18 column was used for the separation of selected compounds in wheat extract with water-acetonitrile-methanol (82:9:9,v/v/v) at a flowrate of 1 mL/min without a split.  Calibration curves showed good linearity and reproducibility.  NIV and DON could by detected down to ug/kg level in wheat using selected ion monitoring of the [M-H] ions and the main fragments.