บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Rapid deter mination of citrinin in corn by fluores cence liquid chromatography and enzyme immunoassay.

ABRAMSON, D., USLEBER, E. and MARTLBAUER, E.

Journal of AOAC International 82: 1353 – 1356.

1999

บทคัดย่อ

Rapid deter mination of citrinin in corn by fluores cence liquid chromatography and enzyme immunoassay.

A rapid  assay procedure was developed for citrinin in corn using liquid – liquid extraction (LLE) cartridges.  Ground corn was extracted with methylene chloried and phosphoric acid and the extract added to an LLE cartridge containing a diatomaceous earth adsorbant, previously impregnated with sodium bicarbonate solution.  After aspiration to dryness, the cartridge was eluted with methanol – water (4 + 1) and aliquots were taken for quanification by reversed phase LC with fluorescence detection. Recoveries of citrinin added to ground corn at 200 – 1600mg/kg ranged from 71.2 to 86.3% with coefficients of variation between 4.1 and 10.6%.  The LLE cartridge procedure offers the advantages of low solvent consumption and speed, and is amenable to automation.