บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Determination of the Fusarium mycotoxins, fusaproliferin and beauvericin by high – performance liquid chromatography – electrospray ionization mass spectrometry.

SEWRAM, V., NIEUWOUDT, T.W., MARASAS, W.F.O., SHEPHARD, G.S. and RITIENI, A.

Journal of Chromatography A 858: 175 – 185.

1999

บทคัดย่อ

Determination of the Fusarium mycotoxins, fusaproliferin and beauvericin by high – performance liquid chromatography – electrospray ionization mass spectrometry.

A method is described using LC-MS for the detection of fusaproliferin and beauvericin in cultures of fusarium subglutinans and in naturally contaminated maize.  Detection of fusaproliferin was best performed in the MS – MS mode while beauvericin displayed a stronger signal in the MS mode.  The on-column instrumental detection limits for pure fusaproliferin and beauvericin were 2 ng ans 20 pg while those in naturally contaminated maize were 1 and 0.5mg/kg, respectively.  Five South African strains of F.subglutinans were analysed following methanol extraction of which four produced fusaproliferin at levels between 330 and 2630 mg/kg while only three produced beauvericin at levels between 140 and 700 mg/kg.  Naturally contaminated maize samples from the Transkei region of South Africa showed fusaproliferin at levels of 8.8 – 39.6 mg/kg and beauvericin at 7.6 – 238.8mg/kg.