บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Rapid immunoaffinity – based method for determination of zearalenone in corn by fluorometry and liquid chromatography.

KRUGER, S.C., KOHN, B., RAMSEY, C.S. and PRIOLI, R.

Journal of AOAC International 82 : 1364-1368.

1999

บทคัดย่อ

Rapid immunoaffinity – based method for determination of zearalenone in corn by fluorometry and liquid chromatography.

An immunoaffinity based method was developed to determine ZEA incorn. Corn samples were extracted in acetonitrile – water (90 + 10, v/v), applied to an immunoaffinity column and eluted with methanol.  The isolated toxin was quantified either by reaction with aluminium chloride either by reaction with aluminium chloride hexahydrate prior to measurement with a  fluorometer or injection into an LC system with a fluorescence detector.  With the immunoaffinity column cleanup procedure,  only ZEA and its metabolites were recognised by the antibody.  Limits of detection were 0.10 mg/kg for the fluorometer and 0.10 or 0.0025 mg/kg (sensitive method) for the LC method.  Percentage recovery averaged 105% (fluorometer) and 93% comparative analysis of 17 naturally contaminated corn samples using Zearala Test LC and the official AOAC LC method for detection of ZEA showed that Zearala Test is statistically comparable to the AOAC Official Method 985.15.