บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Determination of zearalenone in grains by high-performance liquid chromatography tandem mass spectrometry after solid phase extraction with RP-18 columns or immunoaffinity columns

Zollner, P., Jodlbauer, J. and Lindner, W.

Journal of Chromatography A 858 : 167-174.

1999

บทคัดย่อ

Determination of zearalenone in grains by high – perform – ance liquid chromatography – tandem mass spectrometry after solid – phase extraction with RP-18 columns or immunoaffinity columns.

A robust, sensitive and selective LC – MS – MS method for the determination of ZEA in several cereals is described.  Samples were extracted with a mixture of acetonitrile and water followed by solid phase extraction with RP –18 columns or immunoaffinity columns.  The selective de termination of ZEN was achieved with an atmospheric pressure chemical ionization interface.  Using the negative ion mode, a detection limit of 0.5 mg/kg and a determination limit of 1 mg/kg grain was achieved, which is by a factor of 100 more sensitive than the positive ion mode.  A linear working range from 1.0 to 1000 mg/kg could be achieved in grains with a standard deviation of 4% and recovery rates around 100%. All these results were independent from the grain matrices (maize, barley, oats, wheat) when zearalanone was used as internal standard.