บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Quantification of deoxynivalenoi in wheat using an immunoaffinity column and liquid chromatography.

CAHILL, L.M., KRUGER, S.C., MCALICE, B.T., RAMSEY, C.S., PRIOLI, R. and KOHN, B.

Journal of Chromatography A 859 : 23-28.

1999

บทคัดย่อ

Quantification of deoxynivalenoi in wheat using an immunoaffinity column and liquid chromatography.

A simple and accurate method to quantify DON  in wheat which uses immunoaffinity chromatography for DON isolation and LC for toxin detection and quantification is described.  Wheat samples are extracted in water, filtered and applied to an immunoaffinity column.  Following a water wash, DON is eluted from the column with methanol and injected onto a LC system with a UV detector for quantification. Specificity of the immunoaffinity column cleanup procedure was confirmed with only DON and its 15-C derivatives being recognised by the antibody while 3-C DON derivatives, NIV, T-2 and fusarenon X did not bind.  The limit of detection is at least 0.10 mg/kg.  Percentage recovery for the entire assay range averages 90% with an average relative standard deviation of 8.3%. Naturally contaminated samples showed comparable precision.