Immunocytochemical localization of Fusarium toxins in infected wheat spikes by Fusarium culmorum.
KANG, Z. and BUCHENAUER, H.
Physiological and Molecular Plant Pathology 55: 275-288.
1999
บทคัดย่อ
Two antisera raised against DON and 3- acetyl DON were used to investigate the sub-cellular localisation of DON, 3-acetylDON and 15-acetylDON in Fusarium culmorum infected wheat spikes and kernels by means of the immunogold labelling technique. In hyphal cells, the toxins were localised in the cytoplasm, mitochondria, vacuoles and the cell wall. Results suggest that the toxins can be translocated upwards throught the xylem vessels and phloem sieve tubes, and down wards through the phloem sieve tubes. In the infected wheat kernels, the toxins were whown not only in the hyphae, but also in the pericarp tissues, pigment strand, aleurone cells and starchy endosperm in different concentrations.