บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Indirect electrochemical detection of type-B trichothecene mycotoxins.

HSUEH, C.C., LIU, Y. and FREUND, M.S.

Analytical Chemistry 71: 4075-4080.

1999

บทคัดย่อ

Indirect electrochemical detection of type-B trichothecene mycotoxins.

A simple and inexpensive method for the detection of type B trichothecenes is described.  By hydrolysing the toxin under basic conditions at 80° C for 1 hr it is possible to detect the toxin with simple electrochemical techniques.  The detection limit for DON using this method was 9.1 M in solution, corresponding to 0.24 mg/kg in a 25 g grain sample if the final extraction volume is 2.2 mL. The linear dynamic detection range was from 0.32 mg/kg to greater than 32 mg/kg. In rice samples spiked with DON there is no electrochemical interference from rice extract and 1 mg/kg of DON in rice samples can be quantified.  Since the active moiety in DON is common to virtually all type B trichothecenes, this approach may be ideal for type-specific screening.