บทคัดย่องานวิจัย

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Amyl, the alpha-amylase gene of Aspergillus flavus : Involvement in aflatoxin biosynthesis in maize kernels.

FAKHOURY, A.M. and WOLOSHUK, C.P.

Phytopathology 89: 908-914.

1999

บทคัดย่อ

Amyl, the alpha-amylase gene of Aspergillus flavus : Involvement in aflatoxin biosynthesis in maize kernels.

The alpha-amylase gene Amyl was isolated from Aspergillus flavus and its DNA sequence was determined to be nearly identical to Amy3 of A.oryzae. when Amyl was disrupted in an aflatoxigenic strain of A. flavus, the mutant failed to prodeuce extracellular alpha-amylase and grew at 45% the rate of the wild-type strain on starch medium. The mutant produced aflatoxin in medium containing glucose but not in a medium containing starch.  The alpha-amylase-deficient mutant produced aflatoxin in maize kernels with wounded embryos and occasionally produced aflatoxin only in embryos of kernels with wounded endosperm.  The mutant strain failed to produce aflatoxin when inoculated onto degermed kernels.  Results suggest that alpha-amylase facilitates aflatoxin production and growth of A. flavus from a wound in the endosperm to the embryo.