Detecting Aspergillus parasiticus in cereals by an enzyme-linked immunosorbent assay.
TSAI, G.J. and YU, S.C.
International Journal of Food Microbiology. 50 : 181-189.
1999
บทคัดย่อ
An ELISA was used for the specific detection of Aspergillus parasiticus in artificially contaminated corn, rice, wheat and peanut. The growth of A. parasiticus was monitored by plate count and ELISA, and the aflatoxin content was measured. Higher aw levels (aw 0.98 vs. aw 0.92) resulted in higher fungal growth rates and the plate count and ELISA measurements were better correlated, with correlation coefficients of 0.94, 0.93, 0.96 and 0.86 for corn, rice, wheat and peanuts, respectively. Aflatoxin was produced sooner and degraded more rapidly at aw 0.98. Standard plate counting techniques detected A. parasiticus/A. flavus in 5/40 cereal samples bought from retail stores, however ELISA did not give a positive result in any of them. After moisturisation and incubation of these commercial samples at 28 C for 29 days, the incident rates of aflatoxigenic moulds increased to 65% and 52% by plate count and ELISA, respectively.