Comparison of extraction buffers for the detection of fumonisin B1 in corn by immunoassay and high-performance liquid chromatography.
KULISEK, E.S. and HAZEBROEK, J.P.
Journal of Agricutural and Food Chemistry 48 : 65-69.
2000
บทคัดย่อ
A sensitive competitive ELISA using a rabbit polyslonal antibody was developed to secure a rapid and inexpensive screen for FB1 contamination. The assay was used in a comparative study measuring the extraction efficiency of for FB1 in aqueous of organic solvent buffers in 16 field corn samples. An aqueous phosphate buffer was found to be suitable for extracting FB1 ,thus eliminating the need for organic solvents. HPLC and ELISA determinations compared well in fortified samples at known concentrations between 1 and 50 mg/L of extract. Overstimation at levels greater than 50 mg/L were common. The characteristics and application of the ELISA for screening purpose are discussed.