Determination of type A trichothecenes by high-performance liquid chromatography with coumarin-3-carbonyl chloride derivatisation and fluorescence detection.
JIMENEZ, M., MATEO, J.J. and MATEO, R.
Journal of Chromatography A 870: 473-481.
2000
บทคัดย่อ
A method for the analysis of T-2 toxin, HT- toxin, neosolaniol and diacetoxy-scirpenol by HPLC with fluorescence detection using coumarin-3- carbonyl chloride has been developed. For cleanup, liquid-liquid partition between toluene and dihydrogen phosphate buffer at pH was 3-5.5 gave the best results. Separations were performed on a stainless steel LiChrospher 100 C18 reversed phase column with precolumn of the same phase. The mobile phase was acetonitrile-water (65:35) containing 0.75% acetic acid at a flow rate of 1.0 mL/min. The proposed method provides good separation between the four trichothecenes and good reproducibility. The limits of detection were 10 µg/kg for T-2 toxin and about 15 µg/kg for T-2 toxin and about 15 µg/kg for the remaining mycotoxins. The method was applied to the analysis of these mycotoxins in fungal cultures (corn and rice) of Fusarium sporotrichioides, and is also suitable for the quantification of type A trichothecenes in cereals.