Identification of the bright –greenish-yellow-fluores-cence (BGY-F) compound on cotton lint associated with aflatoxin contamination in cottonseed.
ZERINGUE,H.J., SHIH, B.Y., MASKOS, K. and GRIMM, D.
Phytochemistry 52: 1391-1397.
1999
บทคัดย่อ
To characterise the structure of the bright-greenish-yellow-fluorescence (BGY-F) compound on cotton lint associated with aflatoxin contamination in cottonseed, various natural BGY-F reaction products were prepared. BGY-F reaction products prepared from three preparation: (a) kojic acid (KA) + peroxidase + H2O2, (b) detached fresh cotton locules + KA ++ H2O2, and (c) attached field cotton locules that were treated with a spore suspension of aflatoxigenic Aspergillus flavus, all resulted in identical chromatographic characteristics, and all exhibited a molecular weight of 282. Further characterisation by NMR of the BGY-F reaction product revealed that it was a dehydrogenator dimer of 2 KA, linked through the C-6 positions.