บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Cinfirmation of patulin and 5-hydroxymethlfurfural in apple juice by gas chromatograpgy/mass spectrometry.

RUPP, H.S. and TURNIPSEED, S.B.

Journal of AOAC International 83: 612-620.

2000

บทคัดย่อ

Cinfirmation of patulin and 5-hydroxymethlfurfural in apple juice by gas chromatograpgy/mass spectrometry.

A G/c/MS method was developed for the confirmation of patulin and 5-hydroxymethytfurfural (HMF) extracted from apple juice. The extraction is based on the official AOAC method for LC analysis. Juice extracts are quickly and easily derivatised with bis(trimethylsilyl) trifluoracetamide under mild conditions, and the trimethylsislylethers of the analyter are determined by GC/MS using an electron impact source and selected ion monitoring of characteristec ions. The presence of patulin was confirmed at fortification levels of about 30-400 ug/L and naturally occurring levels  of about 80-400 ug/L. The presence of HMF was also confirmed at levels less than or equal to 2 mg/L.