บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Evaluation of silica and sepharosebased immunoaffinity sorbents for sample cleanup in determination of fumonisins B1 and B2 in corn products

Lawrence, J.F., Menard, C., Yeung, J. and Benrejeb, S.

Journal of AOAC International 83:597-603.

2000

บทคัดย่อ

Evaluation of silica and sepharosebased immunoaffinity sorbents for sample cleanup in determination of fumonisins B1 and B2 in corn products.

Anit-FB1 polyclonal antibodies were isolated from the serum of rabbits, immobilised onto the surface of glutaraldehyde-activated silica or Sepharose CL-4B oartuckes abd okaced ubti sikud ogase extractuib cartridges. The immobilised antibodies were evaluated for their abi8lity to retain FB1 and  FB2. The cartrudgesm wgucg were tested fir tgeur effectuvebess to isolate the fumonisins from extracts of corn flour and nacho chips, detected fumonisins down to levels of about 20 ug/kg. With the use of a strong anion exchange cartridge as a preliminary cleanup before immunoaffinity chromatography, the detection limit reached 2-5 ug/kg in the products tested. The silica sorent material exhibited strong interactions with the fumonisins, requiring acidified ethanol-water mixtures for elution and resulting in and additional degree of selectivity in isolating fumonisins from sample extracts. The silica based immunoaffinity cartidges were successfully reused more than 10 times; the Sepharose based cartridges were less robust. LC with fluorescenec detection was used after pre-chromatographic derivatisation with o-phthaldialdehyde-mercaptoethanol.