Effect of tenperature and solvent composition on extraction of fumonisins B1 and B2 from corn products.
Lawrence, J.F., Niedzwiadek, B. and Scott, P.M.
Journal of AOAC International 83: 604-611.
2000
บทคัดย่อ
FB1 and B2 were extracted from naturally contaminated corn products by using different extraction solvent compositions (methanol-water, acetonitrile-methanol-water,of temperatures from ambient to 150°C. Ground samples were mixed with an adsorbent material (hydromatrix(TM)), and the fumonisins were extracted in 2 sequential static extractions at various temperatures. The combined extracts were cleaned up and analysed by reversed phase LC with fluorescence detection after o-phthaldialdehydemercaptoethanol derivatisation. With acetonitrile-methanol-water (1+1+2) thequantity of fumonisins extracted from natrually contaminated taco shells almost tripled in going from 23 to 80°C and increased by another 30% when ethanol-water (3+7) was used as extraction solvent at 80°C. Similar results were obtained with nacho chips. These effects were less pronounced with cornmeal and small differences due to temperature and solvent composition were observed for corn flakes and rice. At 80°C, ethanol-water combinations performed equally or better than methanol-water (8+2) or acetonitrile-methanol-water (1+1+2), combinations which are commonly used for fumonisin extractions. Even 100% water was successful for extracting fumonisins from the products, except for rice. However, increased amounts of water created technical problems and required an increased amount of Hydromatrix in the samples prior to extraction.