Liquid chromatographic method for fumonisin B1 in sorghum syrup and corn-based breakfast cereals.
TRUCKSESS, M.W., CHO, T.H. and READY, D.E.
Food Additives and Contaminants 17: 161-166.
2000
บทคัดย่อ
A method has been diveloped for the determination of FB1 in corn based breakfast cereals and in syrup prepared from sorghum. Test samples were extracted with methanol-0.1 M sodium phosphate buffer (pH3) (1+1). The extract was diluted with water and applied to a 1 gC18 column. The column was washed with acetonitrile-water (2+8). FB1 was eluted with acetonitrile-trifluoroacetic acid (1000+1). The purified extract was evaporated and the toxin was dirivatised with o-phthaldiadehyde-mercaptoethanol, The reaction mixtrue was resoved on a C18 LC colun using acetonitrile-water –acetic acid (5000+550+10.5) as the mobile phase and FB1 measured with a fluorescence detector. Recoveries of FB1 added to samples of sorghum syrup in the range 0.1-1.0 mg/kg were 94-132% Recoveries of FB1 added to samples of breakfast cereal in the range 0.2-1.6 mg/kg were 96-100%