บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

A comparative study of infirect competitive ELISA and HPLC for fumonisin detection in corn of the state of Parana, Brazil.

Ono, E.Y.S., Kawamura, O., Onoj, M.A., Ueno, Y. and Hirooka, E.Y.

Food and Agricultural Immunology 12: 5-14.

2000

บทคัดย่อ

A comparative study of infirect competitive ELISA and HPLC for fumonisin detection in corn of the state of Parana, Brazil.

The performance of a monoclonal antibody based indirect competitive ELISA for fumonisin detection in corn was evaluated using corn samples from the State of Parana, Brazil. Compared to HPLC, the correlation coefficient ® of the results from the two methods was 0.94. The ELISA detected total fumonisin levels of 1.1 to 1.5 fold higher than HPLC in 56.6% of samples. In 13.3% of samples, the ELISA detected 1.6 to 2.0 foled higher values than HPLC. The data show high correlation (r=0.91)of the indirect competitive ELISA and HPLC for fumonisin concentration in corn contaminated with less than or equal to 10 mg/kg. Matrix interference was minimised by diluting the sample before the ELISA assay.