A method for determining fumonisin B1 in corn using immunoaffinity column clean-up and thin layer chromatography/densitometry.
PREIS, R.A. and VARGAS, E.A.
Food Additives and Contaminants 17:463-468.
2000
บทคัดย่อ
A method for determining FB1 in corn was developed and the cleanup optimised in order to give an extract suitable for one-dimensional TLC analysis. FB1 was extracted with a solution of methanol-water (80:20) purified through an immunoaffinity column and separated on a C18 reversed phase TLCplate. The FB1 was visualised with 0.1 mol/L sodium tetraborate, 0.40g/L fluprescamine in acetonitrile and 0.01mol/L boric acid-acetonitrile (2:3) for fluorescence detection and quantified by densitomitric analysis. The mean recovery for FB1 in spiked sample was 85% and the linear equation of standard calibration curve by densitometric. Analysis gave an r(2) value higher than 0.99. The absolute amount of FB1 standard detecable was 2 ng, giving a detection limit for the method of 0.1 mg/kg. In 214 samples of corn collected in different regions of Brazil, FB1 was detected in 99% of samples in the range of 0.2 to 6 mg/kg.