บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Transgenic expression of the TRI101 or PDR5 gene increases resistance of tobacco to the phytotoxic effects of the trichothecene 4,15-diace-toxyscirpenol.

MUHITCH, M.J., MCCORMICK, S.P., ALEXANDER, N.J. and HOHN, T.M.

Plant Science 157:201-207.

2000

บทคัดย่อ

Transgenic expression of the TRI101 or PDR5 gene increases resistance of tobacco to the phytotoxic effects of the trichothecene 4,15-diace-toxyscirpenol.

The effectiveness of two strategies, metabolic alteration and extracellular transport, to protect plant cells from the deleterious effects of 4,15-diacetoxyscirpenol (DAS) were tested. Tobacco plants were transformed with either the Saccharomyces cerevisiae gene PDR5, which encodes a multidrug transporter, or with the Fusarium sporotrichioides gene  TRI101, which encodes a trichothecene 3-O-acetyltransferase. Both genes conferred significant increased tolerance to lerance to DAS as measured by a sensitive seed germination assay. Expression of PDR5 or TRI101 in a seed specific manner in crop plants such as wheat could lower the incidence of head blight as well as reduce mycotoxin levels within the seed.