Analysis of aberrant virulence of Gibberella zeae following transfornation-mediated complemintation of a trichoth-ecene-deficient (Tri5) mutant.
DESJARDINS, AE., BAI, G.H., PLATTNER, R.D. and PROCTOR, R.H.
Microbiology-UK 146:2059-2068.
2000
บทคัดย่อ
In previous field tests of wheat ear blight, trichothecene non-pfoducing mutants were less virulent than the wild-type pro-genitor strain from which they were derived. Trichothecene producing revertants also were restored to wild-type levels of virulence. In contrast, in the field test of wheat ear blight reported khere, trichothecene producing strains obtained by Tri5 mutant complementation were not restored to wild-type levels of virulence. The complemented mutants showed a slightly reduced radial growth compared to the wild-type strain morphology, pigmentation and sexual fertility. Genetic analysis indicated that the aberrant virulence of a complemented mutant was likely due to non-target effects that occurred during the process of transforming the trichothecene non-producing mutant with Tri5.