บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Natural deoxynivalenol (DON)contamination of wheat samples grown in 1996 as deternined by high-performance liquid chromatography.

Fazekas, B., Hajdu, E.T., Tar., A.K. and Tanyi, J.

Acta Veterinaria Hungarica 48:515-160.

2000

บทคัดย่อ

Natural deoxynivalenol (DON)contamination of wheat samples grown in 1996 as deternined by high-performance liquid chromatography.

An HPLC-diode array detection (DAD) method was develiped for determining the DON content of wheat and other cereals. Samples were ectracted with a mixture of acetonitrile and water (84+16). Part of the extract was evaporated and purified on Florisil and activated charcoal colimns. HPLC separation was perrormed on a C18 column, using acetonitrle-water (8+92) as eluent. DAD was performed at 218 and 236 nm by determination of the UV spectrum. The recovery rate of DON was 75% and the detection limit was 0.05 mg/kg. Using this method, the DON content of feeding wheat samples grown in Hungary was determined. DON contamination of wheat was of higher  prevalence (100%) and severity (0.27-4.3 mg/kg) in the southeastern county of Bekes than in Szabolcs county located in the northeastern   part of Hungary (82% and 0.05-1.3 mg/kg).