In vitro degradation of aflatoxin B1 in groundnut (Arachis hypogea) meal by horse radish peroxidase.
DAS, C. and MISHRA, H.N.
Lebensmittel Wissenschaft und – Technologie 33:308-312.
2000
บทคัดย่อ
Finely powdered defatted groundnut meal spiked with AFB1 was treated with commercial horseradish peroxidase and partially purified peroxidase enzyme from freshly harvested radish roots. The optimum enzyme concentration occurred when 10 IU of enzyme dissolved in 50 mM phosphate buffer at pH 6 was mixed homogeneously with 100 g of groundnut meal to react upon 1.4 mM AFB1 at 20° C and mormal pressure up to 24 hr incubation period. Hydrogen peroxide in 20 mM concentration was used as an oxidising agent in all the reactions performed. Moisture content of the reaction media was maintained at 12-15%