บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Determination of ergovaline in endophyted seeds by high performance thin layer chromatography (HPTLC).

VIDEMANN, B., BONY, S. and BERNY, P.

Journal of Liquid Chromatography & Related Technologies 23: 2727-2738.

2000

บทคัดย่อ

Determination of ergovaline in endophyted seeds by high performance thin layer chromatography (HPTLC).

An HPTLC method for the rapid determination of ergovalin in seeds of Graminacecae infested with Neotyphodium coenophialum is described. Quinine is used as an internal standard. The mycotoxin is solubilised under acid conditions and extracted with chloroform under alkaline conditions. Samples are sprayed on silicagel plated and detected by fluorescence. The method developed is specific for endophyte infested seeds, linear from 3 to 12 mg/kg and is both repeatable and reproducible. The limit of detection is 0.5 mg/kg and the limit of determination is 3.0 mg/kg