บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Liquid chromatographic method for determination of patulin in clear and cloudy apple juices and apple puree: Collaborative study.

MACDONALD, S., LONG, M., GILBERT, J. and FELGUEIRAS, I.

Journal of AOAC International 83: 1387-1394.

2000

บทคัดย่อ

Liquid chromatographic method for determination of patulin in clear and cloudy apple juices and apple puree: Collaborative study.

A collaborative trial was conducted to validate the effectiveness of a LC procesure for determination of patulin in both clear. And cloudy apple juices and apple puree. The test portion of clear apple juice was directly extracted with ethyl acetate. Cloudy apple juice and apple puree were treated with pectinase  enzyme before extraxtion. After back-extraction into sodium carbonate to remove interfering acidic compounds, the extract was dried and concentrated, and patulin was determined by LC with UV detection. For the three sample types spikes with patulin at 75 mg/kg, recoveries ranged from 80 to 92%. The relative standard deviations for repeatability (RSDr) and reproducibility (RSDR) ranged from 8 to 35% and 11 to 36%, respectively.