บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Determination of patulin in apple juice by high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.

SEWRAM, V., NAIR, J.J., NIEUWOUDT, T.W., LEGGOTT, N.L. and SHEPHARD, G.S.

Journal of Chromatography A897: 365-374.

2000

บทคัดย่อ

Determination of patulin in apple juice by high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.

An HPLC-MS-MS method with selected reaction monitoring for the determination of patulin in apple juice samples is described. MS detection was accomplished following atmospheric pressure chemical ionisation in both positive and negative ion mode proved superior and a linear response was accomplished following atmospheric pressure chemical ionisation in both positive and negative ion medes. Detection in the negative ion mode proved superior and a linear response was observed over the injected range from 6 to 200 ng patulin. Apple juice samples spiked with patulin between 10 and 135 mg/L were analysed following liquid-liquid extraction with ethyl acetate and cleanup with sodium carbonate. Utilising reversed phase HPLC with acetonitrile-water (10:90), levels down to 10 mg/L were readily quantified and a detection limit of 4 mg/L was attainable. The MS data for the spiked samples compared well to the UV data.