บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Screening of commercial hydrolases for the degradation of ochratoxin A.

STANDER, M.A., BORNSCHEUER, U.T., HENKE, E. and STEYN, P.S.

Journal of Agricultural and Food Chemistry 48. 5736-5739.

2000

บทคัดย่อ

Screening of commercial hydrolases for the degradation of ochratoxin A.

A number of commercial hydroleses were screened for the ability to degrade OA to nontoxic compounds. A crude lipase from Aspergillus niger (Amano A) proved to substantially hydrolyse OA to the nontoxic alphaO and phenylalanine, as confirmed by HPLC with fluorescence detection. The enzyme was purified by anion exchange chromatography to homogeneity. Activity staining of the purified enzyme with a-naphthyl acetate/Fast Red revealed only one band exhibiting hyfrolytic activity. The specific activity of the purified enzyme toward OA was 2.32 units/mg.