บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Liquid chromatographic method with immunoaffinity column cleanup for determination of ocheatoxin A in barley: Collaborative study.

ENTWISLE, A.C., WILLIAMS, A.C., MANN, P.J., SLACK, P.T. and GILBERT, J.

Journal of AOAC International 83. 1377-1383.

2000

บทคัดย่อ

Liquid chromatographic method with immunoaffinity column cleanup for determination of ocheatoxin A in barley: Collaborative study.

A collaborative study was conducted to evaluate a LC method with immunoaffinity column cleanup for determination of OA. The test portion was extracted with acetonitrile-water by blending at high speed. The extract was filtered, diluted with phosphatebuffered saline and applied to an OA immunoaffinity column. The column was washed with water and the OA eluted with methanol. The solvent was then evaporated and the residue redissoloved in injection solvent. After injection of this solution onto reversed phase LC column, OA was measured by fluorescence detection. Test portions were spiked with OA at 4 mg/kg and recoveries ranged from 65 to 113%. The relative standard deviation for repeatability (RSDr) ranged from 4 to 24%, and the relative standard deviation for reproducibility (RSDR) ranged from 12 to 33%.