Production of polyclonal antibodies against ochratoxin A and its detecgtion in chilies by ELISA.
THIRUMALA-DEVI, K., MAYO, M.A., REDDY. G., REDDY. S.V., DELFOSSE, P. and REDDY. D.V.R.
Journal of Agricultural and Food Chemistry 48: 5079-5082.
2000
บทคัดย่อ
Polyclonal antibodies were produced for OA by injecting OA-boving serum albumin conjugate sc into a New Zealand White inbred rabbit. Antiserum could be used at a dilution exceeding 1:100 000 in an indirect competitive ELISA, and detected OA concentrations up to 0.1 mg/L. Antibodied did not react with OB, coumarin, 4-hydroxysoumarin, L-phenylalanine or AFB1. OA contamination in chilies (Capsicum annum L.) collected from commercial markets and cold storage units was determined. Of 100 chili samples tested, 26 were found to contain over 10 mg/kg of OA. This is the first record in India of OA in chilies, a major component of cooked foods in this country, and it is noteworthy that OA contamination exceeded the permissible limit for human consumption (< 20 mg/kg) in over 26% of the market samples tested.