Monoclonal antibodies for the mycotoxins deoxynivalenol and 3-acetly-deoxyniyalenol.
MARAGOS, C.M. and MCCORMICK, S.P
Food and Agricultural Immunology 12: 181-192.
2000
บทคัดย่อ
Three monoclonal antibodies were developed following the immunisation of mice with a conjugate of DON and ovalbumin. One of these antibodies was selected for the development of a competitive direct ELISA (CD-ELISA). The IC50 for colour development in the assay for DON was 18 mg/L. The antibody from this clone showed strong cross-reactivity to 3-acetlDON, with an IC50 of 2.9 mg/L. Cross-reactivity to 19 other DON over the range 0.01-10 mg/kg. Recoveries over the range 0.05-5 mg/kg averaged 88.7% with a coefficient of variation of 10.9%.