บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Novel procedure for identification of compounds inhibitory to transcription of genes involved in mycotoxin biosynthesis.

ANNIS, S.L., VELASQUEZ, L., XU, H.X., HAMMERSCHMIDT, R., LINZ, J. and TRAIL, F.

Journal of Agricultural and Food Chemistry48: 4656-4660.

2000

บทคัดย่อ

Novel procedure for identification of compounds inhibitory to transcription of genes involved in mycotoxin biosynthesis.

A novel assay is described for the identification and isolation of compounds that inhibit the transcription of genes involves in mycotoxin biosythesis. The TLC based assay was used to screen plant extracts for compounds that would inhibit the expression of the beta-glucuronidase reporter gene under the control of an aflatoxin biosynthesis gene promoter in Aspergillus parasiticus. The assay was used to track purification of an inhibitory compound, cp2, from extracts of blck pepper (Piper nigrum). Cp2 inhibited aflatoxin biosynthesis at the transcriptional level.