บทคัดย่องานวิจัย

Deoxynivalenol and ochratoxin A in German wheat and changes of level in relation to storage parameters.

BIRZELE, B., PRANGE, A. and KRAMER, J.

Food Additives and Contaminants 17: 1027-1035.

2000

บทคัดย่อ

Deoxynivalenol and ochratoxin A in German wheat and changes of level in relation to storage parameters.

The  occurrence  of  deoxynivalenol (DON) and  ochratoxin A (OA)  in  the  winter wheat of  1997  and  1998 grown under  organic  farming  conditions  was  investigated.  The  influence  of  delaysed  drying  of  the  grain  after  harvest  on  the  development  of  DON and  OA  was determined  in  storage  trials. The  Tox5  PCR  assay was  used  both  to detect  Fusarium  species  with  the  potential  to  produce  trichothecenes  and  as  a measure  of their  relative DNA content  during  the  storange  trials.  The  intensity of the  PCR  signals  was  correlated with  the DON  concentration.  All  the  freshly  harvested  grain  samples  were  contaminated with  DON  and  showed  further increases  in  the DON  concentration during  storage.  OA  contamination was  found  in 14.3%  of  the  1997  samples and  in 24.1%  of  the  1998  samples.  OA  increased during  storage  trials  of   the  1997  samples  but  not  in  the  1998  samples.