บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Detection of T- 2 toxin in different cereals by flow – through enzyme immunoassay with a simultaneous internal reference.

SIBANDA, L., DESAGER, S.,VANPETEGHEM, C., GRABARKIEWICZ – SZCZESNA, J. and TOMCZAK, M.

Journal of Agricultural and Food Chemistry 48: 5864-5867.

2000

บทคัดย่อ

Detection of T- 2 toxin in different cereals by flow – through enzyme immunoassay with a simultaneous internal reference.

In most previously described membrane based immunoassays a separate negative control assay is carried out to evaluate the performance of the assay. To overcome this problem, a membrane based flow – through enzyme immunoassay with an internal control has been developed for the detection of T-2 toxin in cereals. An Immunodyne ABC membrane was coated with goat anti – horse – radish peroxidase (HRP) (internal control spot) and rabbit anti-mouse (test spot) immunoglobulins, and the free bindign sites were blocked. In addition to the antibody – coated Immunodyne ABC membrane, the assay also comprises a plastic snap – fit device, absorbent cotion wool, mouse anti T-2 monoclonal antibodies, and T-2 HRP conjugate. The minimum detectable limit for T-2 toxin was 50 mg/kg of cereal sample.