Determination of fumonisins B1 and B2 in cornflakes by high performance liquid chromatography and immunoaffinity clean – up.
SOLFRIZZO, M., DEGIROLAMO, A. and VISCONTI, A.
Food Additives and Contaminants 18: 227 – 235.
2001
บทคัดย่อ
An accurate method for the determination of FB1 and B2 in cornflakes has been developed. Samples were extracted with acetonitrile – methanol – water (25: 25: 50), diluted with phosphate buffered saline (PBS) and applied to a FumoniTest ™ immunoaffinity column. After washing with PBX, fumonisins were eluted from the column with methanol and reacted with o-phthaldialdehyde / 2 – mercaptoethanol to form fluorescent derivatives. Fumonisin derivatives were analysed by reversed phase HPLC with fluorometric detection. The average recoveries for FB1 and FB2 spiked in the ranges 0.33 – 2.80 and 0.17 – 1.40 mg/kg were 102.6 and 95.1%, respectively, with average relative standard deviations of 9 and 8%, respectively. The limit of quantification for FB1 and FB2 was 0.005 mg/kg. In 18 cornflakes and cornflake cereals samples tested, all but one sample were found to be contaminated, with mean concentrations of FB1 and FB2 of 0.157 and 0.036 mg/kg, respectively.