บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Quantification of trichothecene – producing Fusarium species in harvested grain by competitive PCR to determine efficacies of fungicides against Fusarium head – blight of winter wheat.

EDWARDS, S.G., PIRGOZLIEV, S.R., HARE, M.C. and JENKINSON, P.

Applied and Environmental Microbiology 67: 1575 – 1580.

2001

บทคัดย่อ

Quantification of trichothecene – producing Fusarium species in harvested grain by competitive PCR to determine efficacies of fungicides against Fusarium head – blight of winter wheat.

A PCR based assay to quantify trichothecene producing Fusarium based on primers derived from the trichodiene synthase gene (Tri5) is described. The primers were tested against a range of FHB pathogens and found to amplify specifically a 260 – bp product from 25 isolates belonging to six trichothecene producting Fusarium species. Amounts of the trichothecene – producting Fusarium and DON in harvested grain from a field trial designed to test the efficacies of the fungicides metconazole, azoxystrobin and tebuconazole to control FHB were quantified. No correlation was found between FHB severity and DON in harvested grain, but a good correlation existed between the amount of trichothecene – producing Fusarium and DON present within grain. Azoxystrobin did not affect levels of trichothecene – producting Fusarium compared with controls. Metconazole and tebuconazole significantly reduced the amount of trichothecene producing Fusarium in harvested grain.