บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Critical study of and improvements in chromatographic methods for the analysis of type B trichothecenes.

MATEO, J.J., LLORENS, A., MATEO, R. and JIMENEZ, M.

Journal of Chromatography A918: 99 – 112.

2001

บทคัดย่อ

Critical study of and improvements in chromatographic methods for the analysis of type B trichothecenes.

Verious analytical methods used in the analysis of type B trichothecenes (DON, NIV, 3- and 15-acetylDON) in cereals were compared and optimised. The extraction solvent of choice was a mixture of acetonitrile – water ( 84 : 16, v/v). The MycoSep 225 column was chosen as the best alternative for cleanup of grain samples. For GC – electron capture detection analysis, derivatisation of analytes with heptafluorobutyric anhydride prior the final determination was chosen as the most suitable procedure, HPLC – photodiode array (at 221 nm) analysis was more suitable than HPLC of the fluorescent coumarin – 3 – carbonyl derivatives. Recoveries obtained in spiked corn, rice and wheat are reported.

Tricothecenes – Toxicoses